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Image Search Results
Journal: Cell Reports Medicine
Article Title: Treatment effects of soluble guanylate cyclase modulation on diabetic kidney disease at single-cell resolution
doi: 10.1016/j.xcrm.2023.100992
Figure Lengend Snippet: Diabetic ZSF1 rats recapitulate phenotypic changes of DKD with marked disease improvement by sGC activators (A) Representation of the importance of heme-containing (native) sGC and heme-free (dysfunctional) form of sGC and its redox equilibrium. sGC stimulator efficacy depends on the ferrous, Fe(II), state of the heme group at the β subunit of sGC, while sGC activators bind directly to oxidized, Fe(III), or heme-free apo form of sGC. Similar to other cardiovascular disorders, DKD is associated with reduced NO bioavailability, increased oxidative stress, and endothelial dysfunction. cGMP, cyclic guanosine monophosphate; DKD, diabetic kidney disease; NO, nitric oxide; NOS, nitric oxide synthase; O 2 − , superoxide; ONOO − , peroxynitrite; sGCact, soluble guanylate cyclase activator; sGCstim, soluble guanylate cyclase stimulator. Adapted from Sandner et al. (B) Experimental ZSF1 rat model setup. sGCact, soluble guanylate cyclase activator; sGCstim, sGC stimulator. (C–E) Metabolic (serum cholesterol, glucose, and plasma HbA1c) (C), kidney function (D), and kidney injury markers (E) after 12 study weeks; p values are given for either one-way ANOVA or Kruskal-Wallis test (both Benjamini, Krieger, Yekutieli corrected). Ob, obese; ns, not significant. Color legend as in (B). (F) Histopathology changes in hematoxylin/eosin (left) and Sirius red/fast green (right) stained kidney sections. Scale bars, 500 μm. (G) Histopathology scoring; p values are given for Kruskal-Wallis test (Benjamini, Krieger, Yekutieli corrected). Color legend as in (B).
Article Snippet: With regard to biomarkers, all assays were performed according to manufacturers’ instructions: Plasma KIM-1 and neutrophil gelatinase-associated lipocalin (NGAL) were measured using a
Techniques: Clinical Proteomics, Histopathology, Staining